CD34+Stem Cell Selection for Patients Receiving Partially Matched Family or Matched Unrelated Adult Donor Allogeneic Stem Cell Transplantations for Malignant and Non-Malignant Disease

Inclusion Criteria:

- Adequate renal function defined as:Serum creatinine <1.5 x normal, or Creatinine
clearance or radioisotope GFR >60 ml/min/m2 or >60 ml/min/1.73 m2 or an equivalent
GFR as determined by the institutional normal range.

- Adequate liver function defined as:Total bilirubin <1.5 x normal, or SGOT (AST) or
SGPT (ALT) <3.0 x normal

- Adequate cardiac function defined as:Shortening fraction >27% by echocardiogram, or
Ejection fraction of >47% by radionucleotide angiogram or echocardiogram.

- Adequate pulmonary function defined as:Uncorrected DLCO >50% by pulmonary function
test. For children who are uncooperative, no evidence of dyspnea at rest, no exercise
intolerance, and a pulse oximetry >94% on room air.

Eligibility for Reduced Intensity Regimen:

- Adequate renal function defined as:Serum creatinine 2.0 x normal, or creatinine
clearance or radioisotope GFR > 40 ml/min/m2 or >40 ml/min/1.73 m2 or an equivalent
GFR as determined by the institutional normal range.

- Adequate liver function defined as:Total bilirubin < 2.5 x normal; or SGOT (AST) or
SGPT (ALT) < 5.0 x normal.

- Adequate cardiac function defined as:Shortening fraction of >25% by echocardiogram,
or Ejection fraction of >40% by radionuclide angiogram or echocardiogram.

- Adequate pulmonary function defined as:DLCO >35% by pulmonary function test. For
children who are uncooperative, no evidence of dyspnea at rest, no exercise
intolerance, and a pulse oximetry >94% in room air.

Exclusion Criteria:

- Pregnancy/Breast Feeding: Females who are pregnant or breast-feeding are not
eligible.

- Infection: Patients with documented uncontrolled infection at the time of study entry
are not eligible.