Establishing Continuous Cell Lines and Xenografts From Pediatric Cancers for Biological and Pre-Clinical Therapeutic Studies
N/A
21 Years
Both
Cancer
Trial Information
Establishing Continuous Cell Lines and Xenografts From Pediatric Cancers for Biological and Pre-Clinical Therapeutic Studies
OBJECTIVES:
- Establish and bank cell lines and/or xenografts from pediatric patients with cancer.
- Establish continuous cell lines, under carefully controlled conditions, from pediatric
patients with cancer.
- Establish transplantable xenografts in immunocompromised mice from tumor cells that are
difficult to establish as continuous cell lines in vitro.
- Create a bank of cell lines and generate sufficient vials of cryopreserved cells for
distribution to investigators with approved COG biology protocols.
- Characterize cell lines from childhood cancers with respect to DNA short tandem repeat
molecular profile as a "fingerprint" of original cell line identity.
- Characterize cell lines for the ability for sustained growth in tissue culture and/or
as mouse xenografts.
- Characterize cell lines for mycoplasma contamination.
- Characterize cell lines for expression of molecular makers that confirm the tumor-type
of the cell line and the immortal nature of the cells (telomerase) and the expression
of molecular markers that may correlate with drug resistance.
OUTLINE: This is a multicenter study. Specimens are stratified according to disease (acute
lymphoblastic leukemia vs acute myeloid leukemia vs lymphoma vs osteogenic sarcoma vs Ewing
family of tumors vs rhabdomyosarcoma vs primitive neuroectodermal tumor vs glioma vs
astrocytoma vs rhabdoid tumors vs hepatoblastoma vs retinoblastoma vs Wilms tumor vs germ
cell tumors vs other diagnoses).
Leftover tissue from diagnostic procedures and/or surgery is cryopreserved and banked. Blood
and/or bone marrow are also collected and banked.
Cell lines are established and characterized via reverse-transcriptase polymerase chain
reaction and/or flow cytometry for biomarkers and by DNA fingerprinting.
Markers to be identified may include the following:
- Neuroblastoma: tyrosine hydroxylase, protein gene product (PGP) 9.5, GD2, HLA class I,
and HSAN 1.2 antigens
- Ewing family of tumors: EWS-FLI1, EWS-ERG, and PGP 9.5
- Retinoblastoma: interphotoreceptor retinoid-binding protein
- Acute lymphoblastic leukemia: immunophenotype
- Alveolar rhabdomyosarcoma: PAX3-FKHR, PAX7-FKHR, and MyoD1
- All cell types: telomerase expression including hTR and hTERT Mutations of TP53 gene
are detected by flow cytometry and/or immunocytochemistry.
No results of these tests are provided to the patient, the patient's physician, or the
patient's medical records.
PROJECTED ACCRUAL: A total of 500 specimens per stratum will be accrued for this study.
Inclusion Criteria
DISEASE CHARACTERISTICS:
- All malignant tissues from childhood cancers allowed including the following:
- Brain tumors (all types)
- Tissue should be submitted to CNS Committee Resource labs to be forwarded
for this study, unless instructed otherwise on the COG web site
- Ewing family of tumors
- Rhabdomyosarcomas
- Other soft tissue sarcomas
- Osteogenic sarcomas
- Rhabdoid tumors
- Neuroblastomas
- Viable material for cell culture for neuroblastoma is collected via
COG-ANBL00B1 and should not be submitted via this study unless the patient
cannot be enrolled on COG-ANBL00B1* NOTE: *The same applies to any similar
biology studies from other disease committees
- Retinoblastomas
- Anaplastic Wilms tumor
- Germ cell tumors
- Leukemias/lymphomas
- Acute myeloid leukemia (AML)
- Blood samples and bone marrow samples from patients at second relapse
and beyond may be submitted for this study
- Bone marrow samples at diagnosis or first relapse must be submitted to
an AML resource lab and will be forwarded for this study at the
discretion of the AML Committee
- Acute lymphoblastic leukemia (ALL)
- Blood samples may be submitted directly to this study
- Bone marrow samples must be submitted to an ALL resource lab and will
be forwarded for this study at the discretion of the ALL Committee
- Enrolled on a COG therapeutic, biology, or tissue banking protocol that allows
collection of tissue for research and submission to a COG-designated resource
laboratory
- Participation in this protocol is not permitted until after tissue requirements
for any active COG disease-specific therapeutic, biology, or banking protocols
have been satisfied
- Material may only be submitted for this protocol if tissue is available in
excess of that required for satisfying active disease-specific therapeutic and
biological protocols
- Patients with diagnosis pending are eligible
PATIENT CHARACTERISTICS:
- Not specified
PRIOR CONCURRENT THERAPY:
- Not specified
Type of Study:
Observational
Study Design:
N/A
Outcome Measure:
Establishment and banking of cell lines and/or xenografts from pediatric patients with cancer
Safety Issue:
No
Principal Investigator
C. Patrick Reynolds, MD, PhD
Investigator Role:
Study Chair
Investigator Affiliation:
Children's Hospital Los Angeles
Authority:
Unspecified
Study ID:
CDR0000478867
NCT ID:
NCT00898755
Start Date:
March 2007
Completion Date:
Related Keywords:
- Cancer
- childhood acute lymphoblastic leukemia in remission
- unspecified childhood solid tumor, protocol specific
- childhood acute myeloid leukemia in remission
- childhood central nervous system germ cell tumor
- childhood choroid plexus tumor
- childhood chronic myelogenous leukemia
- childhood craniopharyngioma
- childhood diffuse large cell lymphoma
- childhood extragonadal germ cell tumor
- childhood grade III lymphomatoid granulomatosis
- childhood infratentorial ependymoma
- childhood teratoma
- childhood grade I meningioma
- childhood grade II meningioma
- childhood grade III meningioma
- childhood supratentorial ependymoma
- childhood high-grade cerebral astrocytoma
- childhood low-grade cerebral astrocytoma
- metastatic childhood soft tissue sarcoma
- newly diagnosed childhood ependymoma
- nonmetastatic childhood soft tissue sarcoma
- previously treated childhood rhabdomyosarcoma
- previously untreated childhood rhabdomyosarcoma
- recurrent childhood acute lymphoblastic leukemia
- recurrent childhood acute myeloid leukemia
- recurrent childhood brain stem glioma
- recurrent childhood cerebellar astrocytoma
- recurrent childhood cerebral astrocytoma
- recurrent childhood ependymoma
- recurrent childhood grade III lymphomatoid granulomatosis
- recurrent childhood large cell lymphoma
- recurrent childhood lymphoblastic lymphoma
- recurrent childhood malignant germ cell tumor
- recurrent childhood medulloblastoma
- recurrent childhood rhabdomyosarcoma
- recurrent childhood small noncleaved cell lymphoma
- recurrent childhood soft tissue sarcoma
- recurrent childhood supratentorial primitive neuroectodermal tumor
- recurrent childhood visual pathway and hypothalamic glioma
- recurrent Wilms tumor and other childhood kidney tumors
- recurrent/refractory childhood Hodgkin lymphoma
- stage I childhood Hodgkin lymphoma
- stage I childhood large cell lymphoma
- stage I childhood liver cancer
- stage I childhood lymphoblastic lymphoma
- stage I childhood small noncleaved cell lymphoma
- stage II childhood Hodgkin lymphoma
- stage II childhood large cell lymphoma
- stage II childhood lymphoblastic lymphoma
- stage II childhood small noncleaved cell lymphoma
- stage III childhood Hodgkin lymphoma
- stage III childhood large cell lymphoma
- stage III childhood lymphoblastic lymphoma
- stage III childhood small noncleaved cell lymphoma
- stage IV childhood Hodgkin lymphoma
- stage IV childhood large cell lymphoma
- stage IV childhood lymphoblastic lymphoma
- stage IV childhood small noncleaved cell lymphoma
- untreated childhood acute lymphoblastic leukemia
- untreated childhood acute myeloid leukemia and other myeloid malignancies
- untreated childhood brain stem glioma
- untreated childhood cerebellar astrocytoma
- untreated childhood medulloblastoma
- untreated childhood supratentorial primitive neuroectodermal tumor
- untreated childhood visual pathway and hypothalamic glioma
- childhood hepatoblastoma
- extraosseous Ewing sarcoma/peripheral primitive neuroectodermal tumor
- localized Ewing sarcoma/peripheral primitive neuroectodermal tumor
- metastatic Ewing sarcoma/peripheral primitive neuroectodermal tumor
- recurrent Ewing sarcoma/peripheral primitive neuroectodermal tumor
- childhood hepatocellular carcinoma
- childhood immunoblastic large cell lymphoma
- childhood malignant ovarian germ cell tumor
- childhood malignant testicular germ cell tumor
- childhood Langerhans cell histiocytosis
- alveolar childhood rhabdomyosarcoma
- childhood Burkitt lymphoma
- de novo myelodysplastic syndromes
- previously treated myelodysplastic syndromes
- secondary myelodysplastic syndromes
- disseminated neuroblastoma
- localized resectable neuroblastoma
- localized unresectable neuroblastoma
- recurrent neuroblastoma
- regional neuroblastoma
- stage 4S neuroblastoma
- childhood germ cell tumor
- recurrent childhood liver cancer
- localized osteosarcoma
- metastatic osteosarcoma
- recurrent osteosarcoma
- extraocular retinoblastoma
- intraocular retinoblastoma
- recurrent retinoblastoma
- stage I Wilms tumor
- stage II Wilms tumor
- stage III Wilms tumor
- stage IV Wilms tumor
- stage II childhood liver cancer
- stage III childhood liver cancer
- stage IV childhood liver cancer
- unusual cancers of childhood
- stage V Wilms tumor
- childhood myelodysplastic syndromes
- childhood atypical teratoid/rhabdoid tumor
- rhabdoid tumor of the kidney
- childhood extracranial germ cell tumor
- childhood central nervous system choriocarcinoma
- childhood central nervous system embryonal tumor
- recurrent childhood central nervous system embryonal tumor
- childhood central nervous system germinoma
- childhood central nervous system mixed germ cell tumor
- childhood central nervous system teratoma
- childhood central nervous system yolk sac tumor
- Lymphoma, Non-Hodgkin
- Neuroectodermal Tumors, Primitive
- Neuroectodermal Tumors, Primitive, Peripheral
Name | Location |
|---|---|
| Presbyterian - St. Luke's Medical Center | Denver, Colorado 80218 |
| Bronson Methodist Hospital | Kalamazoo, Michigan 49007 |
| Ellis Fischel Cancer Center at University of Missouri - Columbia | Columbia, Missouri 65203 |
| Cleveland Clinic Taussig Cancer Center | Cleveland, Ohio 44195 |
| Children's Mercy Hospital | Kansas City, Missouri 64108 |
| All Children's Hospital | St. Petersburg, Florida 33701 |
| Saint Jude Midwest Affiliate | Peoria, Illinois 61637 |
| Driscoll Children's Hospital | Corpus Christi, Texas 78466 |
| City of Hope Comprehensive Cancer Center | Duarte, California 91010 |
| Southern California Permanente Medical Group | Downey, California 90242 |
| Kosair Children's Hospital | Louisville, Kentucky 40202-3830 |
| East Tennessee Children's Hospital | Knoxville, Tennessee 37901 |
| Covenant Children's Hospital | Lubbock, Texas 79410 |
| Arkansas Cancer Research Center at University of Arkansas for Medical Sciences | Little Rock, Arkansas 72205 |
| Blumenthal Cancer Center at Carolinas Medical Center | Charlotte, North Carolina 28232-2861 |
| Carol G. Simon Cancer Center at Morristown Memorial Hospital | Morristown, New Jersey 07962 |
| Jonathan Jaques Children's Cancer Center at Miller Children's Hospital | Long Beach, California 90801 |
| Lee Cancer Care of Lee Memorial Health System | Fort Myers, Florida 33901 |
| St. Joseph's Cancer Institute at St. Joseph's Hospital | Tampa, Florida 33607 |
| Kaplan Cancer Center at St. Mary's Medical Center | West Palm Beach, Florida 33407 |
| Ochsner Cancer Institute at Ochsner Clinic Foundation | New Orleans, Louisiana 70121 |
| Alvin and Lois Lapidus Cancer Institute at Sinai Hospital | Baltimore, Maryland 21215 |
| Hackensack University Medical Center Cancer Center | Hackensack, New Jersey 07601 |
| Wake Forest University Comprehensive Cancer Center | Winston-Salem, North Carolina 27157-1096 |
| Rainbow Babies and Children's Hospital | Cleveland, Ohio 44106-5000 |
| Texas Tech University Health Sciences Center School of Medicine - Amarillo | Amarillo, Texas 79106 |
| Methodist Children's Hospital of South Texas | San Antonio, Texas 78229-3993 |
| Mountain States Tumor Institute at St. Luke's Regional Medical Center | Boise, Idaho 83712-6297 |
| University of Illinois Cancer Center | Chicago, Illinois 60612-7243 |
| Simmons Cooper Cancer Institute | Springfield, Illinois 62794-9677 |
| Lucille P. Markey Cancer Center at University of Kentucky | Lexington, Kentucky 40536-0093 |
| Tulane Cancer Center Office of Clinical Research | Alexandria, Louisiana 71315-3198 |
| CCOP - Nevada Cancer Research Foundation | Las Vegas, Nevada 89109-2306 |
| Herbert Irving Comprehensive Cancer Center at Columbia University Medical Center | New York, New York 10032 |
| Akron Children's Hospital | Akron, Ohio 44308-1062 |
| Tod Children's Hospital | Youngstown, Ohio 44501 |
| Oklahoma University Cancer Institute | Oklahoma City, Oklahoma 73104 |
| Children's Hospital of Pittsburgh of UPMC | Pittsburgh, Pennsylvania 15213 |
| Madigan Army Medical Center - Tacoma | Tacoma, Washington 98431 |
| Connecticut Children's Medical Center | Hartford, Connecticut 06106 |
| BI-LO Charities Children's Cancer Center | Greenville, South Carolina 29605 |
| Children's Hospital of Alabama at University of Alabama at Birmingham | Birmingham, Alabama 35233 |
