A Pilot Trial Of Reduced Intensity Allogeneic Stem Cell Transplantation With Fludarabine, Melphalan, And Low Dose Total Body Irradiation

Inclusion Criteria:

- Diagnosis of a histology documented hematologic malignancy or marrow disorder

- Bone marrow failure disorders and other non-malignant hematologic or immunologic
disorders:

- Acquired bone marrow failure disorders include aplastic anemia, paroxysmal
nocturnal hemoglobinuria (PNH):

- Primary allogeneic hematopoietic stem cell transplantation (HSCT) is
appropriate for selected patients with severe aplastic anemia; however,
patients with aplastic anemia must have failed at least one cycle of
standard immunosuppressive therapy with calcineurin inhibitor plus
anti-thymocyte globulin (ATG) if a fully-matched donor is not available

- Patients with PNH must have a history of thrombosis related to PNH

- Hereditary bone marrow failure disorders include Fanconi anemia or related
chromosomal breakage syndrome dyskeratosis congenita, Diamond-Blackfan anemia,
Shwachman-Diamond syndrome, Kostmann syndrome, congenital amegakaryocytic
thrombocytopenia:

- Fanconi anemia or related chromosomal breakage syndrome: positive
chromosome breakage analysis using diepoxybutane (DEB) or mitomycin C if
applicable

- Dyskeratosis: diagnosis is supported by using either telomerase reverse
transcriptase (TERC) gene mutation in autosomal dominant Dyskeratosis
Congenita or Xlinked DKC1 gene mutation

- Other non-malignant hematologic or immunologic disorders that require
transplantation

- Quantitative or qualitative congenital platelet disorders (including but
not limited to congenital amegakaryocytopenia, absent-radii syndrome,
Glanzmann's thrombasthenia)

- Quantitative or qualitative congenital neutrophil disorders (including but
not limited to chronic granulomatous disease, congenital neutropenia)

- Congenital primary immunodeficiencies (including but not limited to Severe
Combined Immunodeficiency Syndrome, Wiskott-Aldrick syndrome, CD40 ligand
deficiency, T-cell deficiencies)

- Acute leukemias:

- Subjects must be ineligible for conventional myeloablative transplantation;

- Resistant or recurrent disease after at least one standard combination
chemotherapy regime or first remission patients at high risk of relapse OR First
remission patients at high risk of relapse:

- Acute myeloid leukemia (AML)- antecedent myelodysplastic syndrome, secondary
AML, high risk cytogenetic abnormalities or normal cytogenetics with high-risk
molecular features (e.g. Flt3-ITD mutation, mixed-lineage leukemia [MLL],
wildtype NPM1);

- Acute lymphocytic leukemia (ALL)- high or standard risk ALL

- Chronic Myeloid Leukemia (CML):

- Chronic phase (intolerant or unresponsive to imatinib and/or other tyrosine
kinase inhibitors), second chronic phase or accelerated phase who are ineligible
for conventional myeloablative transplantation

- Myeloproliferative and myelodysplastic syndromes (MDS):

- Myelofibrosis (with/without splenectomy) with intermediate to high risk features

- Advanced polycythemia vera not responding to standard therapy

- MDS with an international prostate symptom score (IPSS) score of Int-2 or higher

- MDS with lower IPSS scores Int-1 or less with severe clinical features such as
severe neutropenia or thrombocytopenia or high risk chromosome abnormalities
such as monosomy 7

- Secondary massively parallel signature sequencing (MPSS) with any IPSS scores

- Chronic myelomoncytic leukemia

- Lymphoproliferative disease:

- Chronic lymphocytic leukemia (CLL), low-grade non-Hodgkin lymphoma (NHL)
(recurrent or persistent) fludarabine refractory or with less than 6 months
duration of complete response (CR) between courses of conventional therapy

- Multiple myeloma, progressive disease after autologous stem cell transplant or
as planned tandem (allogeneic transplant after prior autologous stem cell
transplant)

- Waldenstroms macroglobulinemia (failed one standard regimen)

- High grade NHL and diffuse large B-cell lymphoma (DLBCL)

- Not eligible for conventional myeloablative HSCT OR failed autologous HSCT

- First remission lymphoblastic lymphoma, or small, non-cleaved cell lymphoma or
mantle cell lymphoma

- Hodgkin disease:

- Relapsed or refractory after front-line therapy

- Failed or were not eligible for autologous transplantation

- Failed prior autotransplant

- Age >= 3 and =< 75 years for blood and bone marrow transplants and age >= 3, < 60 for
cord blood transplants

- No serious uncontrolled psychiatric illness

- No concomitant active malignancy other than non-melanoma skin cancer

- Non-pregnant and non-nursing women (women or men with reproductive potential should
agree to use an effective means of birth control)

- Patients may have received prior autologous bone marrow transplant (BMT) or prior
myeloablative allogeneic BMT (at least 60 days have elapsed)

- At least 2 weeks since prior chemotherapy, radiation treatment and/or surgery

- Informed consent

- DONOR: Permissible HLA matching: Related donors- single antigen mismatch at HLA A, B
or DRB 1; unrelated donors- a single antigen mismatch at HLA A, B, or C, +/-
additional single allele level mismatch at A, B, C or DRB1; cord blood >= 4 out of 6
antigen match at HLA A, B, DRB1)

- DONOR: Compatibility at the four most informative HLA loci: A, B, C and DRB1 are
important for reducing the risk of GVHD and successful transplant outcomes; the A, B,
C and DRB1 loci comprise 8 possible alleles (a haplotype being inherited from each
parent); one additional locus, HLA-DQ, is also typed to ascertain haplotypes and
assist in the search for a compatible donor; however mismatching at DQ has not been
shown to be associated with adverse outcomes; high resolution molecular typing (at
the allele level) is now the standard of care for unrelated donor searches and allows
greater refinement of the search strategy

- DONOR: Matched related donor: a single antigen mismatch at A, B, or the DR transplant
from a family member is associated with a higher risk of GVHD but similar overall
survival when compared to full identity at these 3 regions; related donor/recipient
pairs must be matched at 5 of 6 HLA antigens (A, B, DRBl)

- DONOR: Unrelated donor: when evaluating patients for unrelated donor transplant, a
higher degree of matching is preferred due to minimize the risk of GVHD; the A, B, C,
DRB1 and DQ loci, comprising 10 possible alleles, will be typed routinely for all
unrelated transplants; given the higher risk of TRM in mismatched transplants, RIT is
often the best way to mitigate the risk; evolving data from the National Marrow Donor
Program now makes it possible to estimate the risks of donor-recipient HLA mismatch
at the allele or antigen level; the higher risk from HLA-mismatching must be
carefully assessed with respect to the clinical urgency and the patient's risk by the
transplant physician; antigen level mismatches at DQ are inconsequential to
transplant outcomes and are ignored with respect to donor selection for the purposes
of this protocol, with matching requirements confined to the 8 loci involving HLA A,
B, C and DRB1; for the purpose of this protocol, a single antigen mismatch at HLA A,
B, or C, with or without additional single allele level mismatch may participate in
this protocol for voluntary unrelated donors (blood or marrow); patients must be at
least antigen-level matched at DRB1

- DONOR: If a patient has no suitable family donor matched for 5 of 6 HLA antigens (A,
B, DRB1) and no suitable unrelated donor is identified or for reasons of urgency, the
patient can be considered a candidate for cord blood transplant, provided a cord
blood donor is identified with a >= 4 out of 6 antigen match at HLA A, B, DRB1
antigens; the cord blood product must provide a minimum of 2 x 10^7 nucleated
cells/kg, test negative for HIV and Hepatitis A, Band C, and sterility assays have no
growth; the cord blood products are located through the National Marrow Donor
Program, the American Registry, or the Bone Marrow Donor Worldwide or other
established registries, and may be stored in the N.Y Placental Cord Blood Bank, the
St. Louis Cord Blood Bank, or any of the established, registered International blood
and marrow banks

- DONOR: Donor must be healthy and have nonreactive test results for all infectious
disease assays as required by state and federal regulations; donors who screen
seropositive for hepatitis and/or syphilis must be cleared by infectious disease
consultation

- DONOR: The donor must have no uncontrolled cardiopulmonary, renal, endocrine, hepatic
or psychiatric disease to render donation unsafe

- DONOR: The donor must be able to give informed consent for peripheral blood stem cell
collection or bone marrow collection

- DONOR: Syngeneic donors are not eligible

- DONOR: Donors who have poor peripheral venous access, may require central venous line
placement for stem cell apheresis

Exclusion Criteria:

- Uncontrolled central nervous system (CNS) disease (for hematologic malignancies)

- Karnofsky (adult) or Lansky (for =< 16 years) performance status =< 50%

- Diffusing capacity of the lung for carbon monoxide (DLCO) less than 40% predicted,
corrected for hemoglobin (Hb) and/or alveolar ventilation

- Cardiac: left ventricular ejection fraction less than 40%

- Bilirubin >= 3 x upper limit of normal

- Liver alkaline phosphatase >= 3 x upper limit of normal

- Serum glutamic oxaloacetic transaminase (SGOT) or serum glutamic pyruvate
transaminase (SGPT) >= 3 x upper limit of normal

- Child's class B and C liver failure

- Calculated creatinine clearance < 40 cc/min by the modified Cockcroft-Gault formula
for adults or the Schwartz formula for pediatrics

- Patients who have received maximally allowed doses (given in 2 Gy fractions, or
equivalent) of previous radiation therapy to various organs as follows:

- Mediastinum 40 Gy

- Heart (any volume) 36 Gy

- Whole lungs 12 Gy

- Small bowel (any volume) 46 Gy

- Kidneys 12 Gy

- Whole liver 20 Gy

- Spinal cord (any volume) 36 Gy

- Whole brain 30 Gy Enrollment of patients who previously receive higher than
allowed dose of radiation to a small volume of lungs, liver, and brain will be
determine by the discretion of the radiation oncologist on the study

- Uncontrolled diabetes mellitus, cardiovascular disease, active serious infection or
other condition which, in the opinion of treating physician, would make this protocol
unreasonably hazardous for the patient

- Human immunodeficiency virus (HIV) positive

- Patients who in the opinion of the treating physician are unlikely to comply with the
restrictions of allogeneic stem cell transplantation based on formal psychosocial
screening

- Females of childbearing potential with a positive pregnancy test